日本精品一区,国产乱视频在线观看,九九九视频,亚洲SSS整片AV在线播放

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-11268 293T/17人胚腎細(xì)胞

CRL-11268 293T/17人胚腎細(xì)胞

簡要描述:CRL-11268 293T/17人胚腎細(xì)胞
,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1621

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CRL-11268 293T/17人胚腎細(xì)胞

Permits and Restrictions

View Restrictions

OrganismHomo sapiens, human
Tissuekidney
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level2 [Cells contain Adeno and SV-40 viral DNA sequences]
Agefetus
Applications

These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.  CRL-11268 293T/17人胚腎細(xì)胞

Storage Conditionsliquid nitrogen vapor phase
Derivation

The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a hmperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned anighly transfectable derivative of the 293 cell line into which the ted the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17.

Antigen Expression

SV40 T antigen

Genes Expressed

SV40 T antigen

Comments

293T/17 cells were cotransfected with the pCRIPenv- and the pCRIPgag-2 vectors to obtain the ANJOU 65 (see ATCC CRL-11269) cell line. ANJOU 65 cells were cotransfected with the pCRIPgag-2 and pGPT2E vectors to obtain the BOSC 23 (see ATCC CRL-11270) ecotropic envelope-expression packaging cell line. ANJOU 65 cells were also cotransfected with the pCRIPAMgag vector along with a plasmid expressing the gpt resistance gene to obtain the Bing (see ATCC CRL-11554)amphotropic envelope-expression packaging cell line.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.      
SubculturingVolumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

S

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品久久久久电影网| 欧美区一区二| 办公室被绑奶头调教羞辱OL | 女同久久精品国产99国产精品| 国产亚洲AV夜间福利香蕉149 | 老司机看片| 欧美性网| 导航| 精品国产欧美一区二区三区成人| 操日本逼| 《乳色吐息》无删减樱花之夜| 成人国产精品日本在线| 高清一区二区三区日本久| 国产午夜福利在线机视频| 久久天天躁狠狠夜夜躁2020| 内地级A艳片高清免费播放| 伊人久久大香线蕉午夜av| 狠狠躁夜夜躁人人爽天天不卡软件 | 中文字幕大看蕉在线观看| 人妻无码久久精品人妻| 亚洲a∨在线播无码av| 亚洲熟女少妇一区二区| 亚洲日本一区二区一本一道| 粗大的内捧猛烈进出视频嘿嘿视频| 亚洲愉拍99热成人精品热久久| 亚洲日本va一区二区三区| 亚洲中文字幕波多野结衣| 2021亚洲va在线va天堂va国产| 亚洲中文字幕aⅴ天堂| 欧美疯狂做受XXXX高潮小说| 久久精品青青大伊人av| 精品成人免费一区二区不卡| 欧美成人WWW免费全部网站| 伊人久久大香线蕉综合av| 把插八插露脸对白内射| 玩弄白嫩少妇xxxxx性| 亚洲日韩中文字幕天堂不卡| 国产永久免费高清在线| 欧美色欧美亚洲高清在线观看| 扒开双腿疯狂进出爽爽爽视频| 精品欧洲av无码一区二区|