日本精品一区,国产乱视频在线观看,九九九视频,亚洲SSS整片AV在线播放

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

簡要描述:Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:1722

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

Panc 04.03 (ATCC® CRL-2555™)

Organism  Homo sapiens, human

Tissue  pancreas

Product Format  frozen

Morphology  epithelial

Culture Properties  adherent

Biosafety Level  1

Disease  adenocarcinoma

Age  70 years adult

Gender  male

Ethnicity  White

Storage Conditions  liquid nitrogen vapor phase

Derivation  Panc 04.03 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.

Clinical Data  70 years

Caucasian

male

Antigen Expression  MHC class I +; MHC class II -

RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Blood type O; Rh+

Oncogene  K-ras + RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Genes Expressed  cytokeratins 7 and 18 RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Tumorigenic  Yes

Effects  Yes, forms tumors in nude or SCID mice

Comments  The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. RefJaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

The cells have a reported plating efficiency of 80%. Ref

Panc 04.03細(xì)胞, 人胰腺癌細(xì)胞

Complete Growth Medium  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

•20 Units/ml human recombinant insulin

•fetal bovine serum to a final concentration of 15%


Subculturing  Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: 1:2 to 1:3

Medium Renewal: Add media once per week. Fluid change one to two times per week.


Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation  Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions  Temperature: 37°C




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
99国产强伦姧在线看RAPE| 日韩精品无码一本二本三本| 日本阿v视频| 中文字字幕码一二三区 | 日本边做边吃奶AⅤ视频免费 | 国产v亚洲v天堂a无码| 热久久国产| 欧美一区二区三区在线播放 | 额济纳旗| 中文字幕第一| 青青久| 国产亚洲制服无码中文| 久久夜色精品国产噜噜| 欧洲国产伦久久久久久久| 精品少妇无码AV在线播放| 亚洲AV中文无码字幕色最| 多毛老太| 欧美人妻精品一区二区三区| 波多野成人无码精品电影| 欧美乱大交XXXXX潮喷| 亚洲国产高清在线观看视频| 亚洲国产精品久久精品| 精品国产免费一区二区三区香蕉 | 久久久人妻| 凤台县| 国产伊人久久| 天堂中文最新版在线官网在线| 欧美a黄片| 国产精品美女WWW爽爽爽视频 | 欧美色综合网| 天天爱天天操| 成年美女黄网站色大免费视频 | 国产成人无码一区二区三区在线 | 国产熟睡乱子伦午夜视频| 一本色道久久综合狠狠躁篇| 日韩人妻少妇一区二区三区 | 日本一级二级三级a| 熟女吧| 午夜三区| 欧美精品欧美精品系列| 色老大|