日本精品一区,国产乱视频在线观看,九九九视频,亚洲SSS整片AV在线播放

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2119HPAC細(xì)胞, 人胰腺癌細(xì)胞

HPAC細(xì)胞, 人胰腺癌細(xì)胞

簡要描述:HPAC細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2119
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-21
  • 訪  問  量:1705

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

HPAC細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2119™    Price:$323.00
Designations:HPACDepositors:WR GowerBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. HPAC細(xì)胞, 人胰腺癌細(xì)胞Isolation:Isolation date: 1985Receptors:epidermal growth factor (EGF), expressed glucocorticoid, expressed epidermal growth factor (EGF); glucocorticoidTumorigenic:YesDNA Profile (STR):Amelogenin: X CSF1PO: 13 D13S317: 11 D16S539: 9,10 D5S818: 12 D7S820: 10,12 THO1: 9.3 TPOX: 10,11 vWA: 15,17Cytogenetic Analysis:modal number = 61Age:64 yearsGender:femaleEthnicity:CaucasianComments:HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin. HPAC proliferation is stimulated by insulin, insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), and transforming growth factor alpha (TGF alpha). Cell growth is suppressed by dexamethasone and other glucocorticoids. HPAC cells are positive for keratin and negative for vimentin and chromogranin A. Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1. Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9. In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells. HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor.Propagation:ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 0.002 mg/ml insulin, 0.005 mg/ml transferrin, 40 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor and 5% fetal bovine serumAtmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                                           Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 3 to 4 daysPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseDoubling Time:41 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006recommended serum:ATCC 30-2020References:22987: Gower WR Jr., et al. HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line. In Vitro Cell. Dev. Biol. 30A: 151-161, 1994.


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
综合无码一区二区三区| 大肉大捧一进一出好爽视频| 丰满岳乱妇在线观看中字| 国产精品视频超级碰| 久9久综合色狠狠综合| 超碰公开在线| 久久精品人人做人人爽电影蜜桃 | 丰满少妇高潮惨叫久久久| 国产性天天综合网| 国产寡妇树林野战在线播放| 无码国产精品一区二区免费3p| 久草影院| xxxx黄色| 国产精品欧美久久久久久| 婷五月天| 亚洲精品入口一区二区在线 | 国产亚洲综合一区二区三区| 延寿县| 保山市| 伊宁县| 嘉义市| 激情内射| 四虎4hu新地址入口2023| 欧美激情图| 人人九九精| 想要导航页| 综综综综合网| 久久久久久免费视频| 我要看黄色一级片| 欧洲区乱码一二三| jizzjizz欧美69巨大| 一级a一级a爱片免费免免在线| 国产浪潮AV性色四虎| 免费无码黄动漫在线观看| 欧美俄罗斯乱妇| 亚洲AV无码成H人动漫在线观看3D| 综合无码一区二区三区| 精品无码日韩一区二区三区不卡| 国产精品久久久久久不卡| 国产精品天干天干综合网| 欧美成人国产精品高潮|