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簡(jiǎn)要描述:CCL-120 CCRF-SD 人外周血淋巴細(xì)胞ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!
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CCL-120 CCRF-SD 人外周血淋巴細(xì)胞
ATCC® Number: CCL-120™ Price: $338.00
Designations: CCRF-SB [CCRF SB]
Depositors: GE Foley
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped
: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human
)
Morphology: lymphoblast
CCL-120 CCRF-SD 人外周血淋巴細(xì)胞
Source: Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: B lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: HLA A1, A2, B12, B17, Cw2
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18
Isoenzymes: G6PD, B
Age: 11.5 years
Gender: male
Ethnicity: Caucasian
Comments: A T cell line (HSB-2, ATCC CCL-120.1) was also obtained from the same buffy coat preparation.
Derived from a buffy coat preparation from a patient with ALL.
The cells are EBNA positive.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 90%; fetal bovine serum, 10% - OR - Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium (10% to 20% by volume) every 2 to 3 days
Related Products: derived from same individual:ATCC CCL-120.1
References: 24413: . M.D. Anderson Hospital and Tumor Inst. Monograph. 21: ; 1967.
25967: Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381
CCL-120 CCRF-SD
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