日本精品一区,国产乱视频在线观看,九九九视频,亚洲SSS整片AV在线播放

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>FL62891人肝細(xì)胞

人肝細(xì)胞

簡(jiǎn)要描述:CRL-11005 FL62891 人肝細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):FL62891
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:2060

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-11005 FL62891 人肝細(xì)胞

Designations: FL 62891
Depositors: ImClone Systems Inc.
Biosafety Level: 2 [Cells contain SV-40 viral DNA sequences ]
Shipped: frozen


Medium & Serum: San)ee PropagationATCC® Number: CRL-11005™ Price: $353.00


Growth Properties: adherent

Organism: Homo sapiens (hum

Morphology: fibroblast

CRL-11005 FL62891 人肝細(xì)胞

Source: Organ: liver

Cell Type: immortalized with SV40 large T antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Age: fetus

Comments: The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 32.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 32°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 48 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993

70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994

70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996

70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997

70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998

70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999



CRL-11005 FL62891



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国内精品九九久久精品| 免费亚洲视频| 久久久久99精品成人片| av一本久道久久波多野结衣| 欧美XXXX做受欧美人妖| 日韩高清在线中文字带字幕| 俺去鲁婷婷六月色综合| 欧美亚洲色综久久精品国产| 国产AV无码亚洲一区二区| 野花在线| 午夜福利在线| 四虎影视网址| 熟妇人妻久久中文字幕| 碰超| 91丨国产丨人妻大屁股| 四虎免费影视| 精品国产一二三| 国产精品久久久久久久女厕留拍| 一木久道热线v| www.夜夜| 欧美a级片| 亚洲爽图| 日韩a区| 国产精品a一区二区三| 国产精品调教| 蜜臀久久精品久久久久 | cn99久久亚洲精品无码毛片| 少妇又色又紧又爽又刺激视频| 婷婷五月在线视频| 深夜放纵内射少妇| 精品人妻少妇一区二区| xxxx丰满少妇高潮| 曰本大码熟中文字幕| 人妻中文av无码。久久| 精品久久久久久无码专区不卡| 亚洲成av人片在www鸭子| 无码内射中文字幕岛国片| 国产精品久久久久电影院| 国产精品无码一二区免费| 日本高清视频www夜色资源| 亚洲精品无码不卡|